First Report of Fig Mosaic Virus on Fig in Russia

HomePlant DiseaseVol. 105, No. 8First Report of Fig Mosaic Virus on in Russia Previous DISEASE NOTE OPENOpen Access licenseFirst RussiaS. Chirkov, S. Tsygankova, Rastorguev, I. Mitrofanova, Chelombit, E. Boulygina, N. Slobodova, and F. SharkoS. Chirkov†Corresponding author: Chirkov; E-mail Address: [email protected]://orcid.org/0000-0002-1353-4373Nikita Botanical Gardens – National Scientific Center, Yalta, 298648, RussiaDepartment Virology, Faculty Biology, Lomonosov Moscow State University, 119234, RussiaSearch for more papers by this author, TsygankovaNational Research Center “Kurchatov Institute”, Moscow, 123182, Rastorguevhttps://orcid.org/0000-0002-0095-0255National MitrofanovaNikita ChelombitNikita BoulyginaNational SlobodovaNational SharkoNational author AffiliationsAuthors Affiliations Chirkov1 2 † Tsygankova3 Rastorguev3 Mitrofanova1 Chelombit1 Boulygina3 Slobodova3 Sharko3 1Nikita 2Department 3National Published Online:18 Aug 2021https://doi.org/10.1094/PDIS-11-20-2463-PDNAboutSectionsView articlePDFPDF PlusSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleFig mosaic virus (FMV) (genus Emaravirus the family Fimoviridae) is considered etiological agent fig disease (FMD), which recorded most fig-growing areas with an average global infection rate 33%. The multipartite FMV genome composed six negative monocistronic ssRNAs, each separately encapsidated (Preising et al. 2021). Although FMD-like symptoms, include mosaic, chlorotic ringspots, oak leaf patterns, were observed approximately a third 400 accessions Nikita Gardens, (Mitrofanova 2016), has not been identified as causal disease. In June 2020, total RNA was isolated from symptomatic leaves 59 trees (32 years old) representing 31 local 27 introduced Ficus carica L. cultivars single pseudocarica Miq. tree using RNeasy Plant Mini kit (Qiagen, U.S.A.). tested reverse transcription PCR primer sets E5 (Elbeaino 2009) EMARAVGP (Walia 2009), amplify 302-bp fragment RNA1 468-bp RNA2, respectively. products expected sizes generated all samples, indicating high incidence plantings. sequences isolates Bleuet, Kraps di Hersh, Smena, Temri determined high-throughput sequencing MiSeq Illumina platform. Double-stranded FMV-positive Viral Gene-spin DNA/RNA Extraction Kit (iNtRON, Korea), followed cDNA library preparation NEBNext Ultra II Library Prep (New England Biolabs, On average, 695,000 quality-filtered 150-bp pair-ended reads per produced used de novo assembly metaSpades program version 3.14 (Nurk 2017). five BLASTn analysis found FMV-related contigs. contigs spanned 99 100% corresponding genomic segments closely related isolates. addition FMV, cryptic virus-related also detected some samples. covering RNA6 had highest identity AM941711 (96.5 96.6%), FM864225 (94.4 94.6%), FM991954 (97.9 98.2%), AB697863 (96.4 AB697879 (93.3 93.4%), AB697895 (95.4 97.0%), Five Russian shared 99.2 nucleotide sequence identity, depending segment. Their deposited GenBank under accession numbers MW201216 MW201230 MW208662 MW208676. Phylogenetic open reading frames (ORFs) showed that ORF1 ORF3 ORF6 clustered Italy, whereas ORF4 grouped isolate JTT-Pa (AB697863) Japan. ORF5 formed separate cluster SB1 SB2 Serbia JTT-Vi Japan (AB697879 AB697884). Incongruency phylogenetic relationship among suggests reassortment ancestors addition, similar SB1, SB2, JTT-Vi, encodes protein 486 amino acid (aa) residues, contrast Italian consisting 502 aa. To best our knowledge, first report Russia. This finding only expands information geographical distribution but extends knowledge natural host virus.The author(s) declare no conflict interest.References:Elbeaino, T., 2009. J. Gen. Virol. 90:1281. https://doi.org/10.1099/vir.0.008649-0 Crossref, ISI, Google ScholarMitrofanova, V., 2016. Acta Hortic. 1139: 303. https://doi.org/10.17660/ActaHortic.2016.1139.53 ScholarNurk, S., 2017. Genome Res. 27:824. https://doi.org/10.1101/gr.213959.116 ScholarPreising, 2021. Dis. 105:727https://doi.org/10.1094/PDIS-06-20-1352-FE Link, ScholarWalia, J., 93:4. https://doi.org/10.1094/PDIS-93-1-0004 ScholarThe interest.Funding: Funding provided Ministry Science Higher Education Federation, grant nos. 075-15-2019-1659 075-15-2019-1670.DetailsFiguresLiterature CitedRelated Vol. 8 August 2021SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionComparison banana plants silicon-deficient soil amended or nonamended calcium silicate infected Fusarium oxysporum f. sp. cubense (W. Zellner al.). Photo credit: A. Fortunato Rodrigues. Infected spicebush outer bark showing black vascular discoloration sapwood typical laurel wilt (R. Olatinwo R. Olatinwo. Maize naturally Bipolaris zeicola (S. Liu Liu. Metrics Downloaded 566 times Article History Issue Date: 1 Dec 2021Published: 18 2021First Look: Jan 2021Accepted: 5 2021 Page: 2260 Information© American Phytopathological SocietyFundingMinistry FederationGrant/Award Number: 075-15-2019-1659Grant/Award 075-15-2019-1670KeywordsFicus L.Ficus Miq.fig diseasefig virushigh-throughput sequencingphylogenyThe interest.